Some botanical gardens in the Czech Republic have collections consisting entirely of Czech Republic native and endemic species; most have a collection that include plants from around the world. Czech Republic botanical gardens and arboreta are administered by local governments, some are privately owned.
List of botanical gardens
- Brno
- Botanical gardens and arboretum of Mendel University of Agriculture and Forestry in Brno
- Botanical gardens of the Faculty of Natural Sciences of Masaryk University
- Hradec Králové, Botanical gardens of medicinal plants of the Pharmaceutical Faculty of Charles University
- Kostelec nad Černými lesy, Arboretum of the Czech Agricultural University in Prague
- Liberec, Liberec botanical gardens
- Olomouc
- Palacký University of Olomouc Botanical gardens
- Olomouc Exhibition Centre of Flora
- Ostrava, Botanical gardens of the Faculty of Natural Sciences of Ostrava University
- Plzeň
- Plzeň zoological and botanical gardens
- Sofronka Arboretum of the Forestry and Game Management Research Institute (VÚLHM)
- Prague
- Praha 2, Na Slupi, Botanical gardens of the Faculty of Natural Sciences of Charles University
- Praha 8, Troja, Botanical gardens of the City of Prague
- Praha 9, Malešice botanical gardens
- Průhonice
- Botanical department of the Czech Academy of Sciences
- Dendrological gardens of the Silva Tarouca Research Institute for Landscape and Ornamental Horticulture
- Rakovník, Botanical gardens of the Rakovník College of Agriculture
- Tábor, Botanical gardens of the Tabor Higher Specialist School and College of Agriculture
- Teplice, Botanical gardens of the City of Teplice